One year passed from the “African-European partnership for development and deployment of rapid SARS-CoV-2 RNA and antigen detection assays”

African-European Partnership for the Development and Deployment of Rapid SARS-CoV-2 RNA and Antigen Detection Assays.

 09, Diaxxo AG

The “African-European partnership for development and deployment of rapid SARS-CoV-2 RNA and antigen detection assays” is part of a joint initiative to bring together research teams from the BRCCH and the European & Developing Countries Clinical Trials Partnership (EDCTP) in collaborative projects.

The research teams, supported by the BRCCH, consist of a combination of scientists from different fields of material science, biotechnology, biology, genetics and medicine. The purpose of this study is the assessment of innovative technologies aimed at controlling the spread of the COVID-19 pandemic.

More specifically, this international consortium is bringing together and validating a variety of testing strategies (including antigen and PCR) in order to identify the solutions that are most suitable for resource-limited settings

During the first half of 2021, the team led by Prof. Stark has successfully developed and deployed multiple devices and several hundred test cartridges for the rapid identification of SARS-Cov-2 by PCR. The reliable production and scale-up of test cartridges has proven particularly challenging, but nevertheless the first batch of test material has been successfully delivered to the African partners in mid-2021. The team is now actively working with the collaborators in Africa in order to assist them in the performance validation, provide technical support and improve the tests for future iterations.

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Covid Variants

diaxxoPod (RUO)

Description

Our in-vitro diagnostic test cartridge based on rapid Polymerase Chain Reaction (PCR) amplification technology. This cartridge can reliably detect and distinguish the mutants from UK (20I/501Y.V1, B.1.1.7 – VOC-202012/01) as well as the South African mutation (B.1.351 lineage).

The test cartridge is aimed to improve the public health situation by rapidly enabling the detection of variants of concern. The cartridge is designed for qualitative detection of variants of interest of the SARS-CoV-2 Viral RNA in saliva samples and it works in conjuction with diaxxoPCR.

Specs

  • Fully preloaded and only require addition of purified sample
  • Results in approx. 30 minutes

Workflow

The test cartridges are sold with all the necessary reagents pre-loaded in the reaction wells. The use of the test cartridges is therefore extremely simple:

  • Apply the sample (viral RNA)
  • Apply water in the control wells and the cover fluid
  • Insert the cartrdige in diaxxoPCR, press a button, drink a coffee and check your results.

Notes & Instructions

  • The cartridges are supplied in a vacuum-sealed bag in order to ensure a long shelf life at room temperature.
  • Detailed instructions about how to use the cartridges and the devices are provided

SARS-CoV-2

diaxxoPod

Description

This SARS-CoV-2 cartridge is based on the US Centers for Disease Control and Prevention (CDC) RT-PCR test for 2019-nCoV. The assay detects 2 parts of the N gene specific for Sars-CoV-2. In addition, an extraction control for Human RNAse P is also included. The cartridge is designed to work in combination with saliva samples and in conjuction with our diaxxoPCR.

Specs

  • Functionally validated and confirmed template-free to cycle 45 by NTC testing
  •  Fully preloaded and only require addition of purified DNA/RNA
  • Results within 30 minutes

Workflow

The test cartridges are sold with all the necessary reagents pre-loaded in the reaction wells. The use of the test cartridges is therefore extremely simple:

  • Apply the sample (viral RNA)
  • Apply water in the control wells and the cover fluid
  • Insert the cartrdige in diaxxoPCR, press a button, drink a coffee and check your results.

Notes & Instructions

  • The cartridges are supplied in a vacuum-sealed bag in order to ensure a long shelf life at room temperature.
  • Detailed instructions about how to use the cartridges and the devices are provided

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