The Swiss National Science Foundation (SNSF) grant for peakPCR research.

Making DNA analysis faster and more accessible

01.06.2020 by SNSF

The Polymerase Chain Reaction (PCR) is a widely used molecular technique to make millions of copy of a DNA sample, with applications in a variety of fields such as medical diagnostics, biotechnology and forensic sciences.During the course of this Bridge – Proof-of-Concept project we have designed a completely new sample holder with extremely high heating and cooling rates and a much lower sample volume, in order to cut analysis time from currently 1-2 h down to 10 minutes, and reagent consumption by 90%. Our sample holder has been engineered in conjunction with a PCR thermocycler platform that is stand-alone and cost effective. Next to a first stand-alone desktop instrument we have also developed a portable version that is battery-powered and that operates in conjunction with a smartphone. We have successfully demonstrated the quantitative PCR capabilities of our platform and we have packaged the technology into a complete ‘Rapid PCR test’.We have also further improved our cartridge also for digital PCR applications and in parallel we have developed a simple and rapid DNA extraction procedure.

The Swiss National Science Foundation (SNSF) grant for peakPCR research.

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Covid Variants

diaxxoPod (RUO)

Description

Our in-vitro diagnostic test cartridge based on rapid Polymerase Chain Reaction (PCR) amplification technology. This cartridge can reliably detect and distinguish the mutants from UK (20I/501Y.V1, B.1.1.7 – VOC-202012/01) as well as the South African mutation (B.1.351 lineage).

The test cartridge is aimed to improve the public health situation by rapidly enabling the detection of variants of concern. The cartridge is designed for qualitative detection of variants of interest of the SARS-CoV-2 Viral RNA in saliva samples and it works in conjuction with diaxxoPCR.

Specs

  • Fully preloaded and only require addition of purified sample
  • Results in approx. 30 minutes

Workflow

The test cartridges are sold with all the necessary reagents pre-loaded in the reaction wells. The use of the test cartridges is therefore extremely simple:

  • Apply the sample (viral RNA)
  • Apply water in the control wells and the cover fluid
  • Insert the cartrdige in diaxxoPCR, press a button, drink a coffee and check your results.

Notes & Instructions

  • The cartridges are supplied in a vacuum-sealed bag in order to ensure a long shelf life at room temperature.
  • Detailed instructions about how to use the cartridges and the devices are provided

SARS-CoV-2

diaxxoPod

Description

This SARS-CoV-2 cartridge is based on the US Centers for Disease Control and Prevention (CDC) RT-PCR test for 2019-nCoV. The assay detects 2 parts of the N gene specific for Sars-CoV-2. In addition, an extraction control for Human RNAse P is also included. The cartridge is designed to work in combination with saliva samples and in conjuction with our diaxxoPCR.

Specs

  • Functionally validated and confirmed template-free to cycle 45 by NTC testing
  •  Fully preloaded and only require addition of purified DNA/RNA
  • Results within 30 minutes

Workflow

The test cartridges are sold with all the necessary reagents pre-loaded in the reaction wells. The use of the test cartridges is therefore extremely simple:

  • Apply the sample (viral RNA)
  • Apply water in the control wells and the cover fluid
  • Insert the cartrdige in diaxxoPCR, press a button, drink a coffee and check your results.

Notes & Instructions

  • The cartridges are supplied in a vacuum-sealed bag in order to ensure a long shelf life at room temperature.
  • Detailed instructions about how to use the cartridges and the devices are provided

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